ABSTRACT Background: Betula platyphylla Sukaczev is one of important tree species due to its ecological and economic value. It is a cold-tolerant tree species which also faces heat stress during summer. In the current study, RNA-Seq profiling of two-month-old B. platyphylla seedlings were conducted utilizing the MGISEQ-2000 platform. Results: In total, 856,347,961 clean reads were obtained from 26 RNA-Seq libraries. Totally, 822,552,820 reads were successfully mapped to B. platyphylla reference genome. Further, a total of 360 and 264 DEGs were discovered under cold and heat exposure, respectively, while a total of 104 DEGs were identified under both cold and heat exposure. It was found that several pathways including response to cold, response to heat, response to temperature stimulus, response to stress, lipid metabolic, jamonic acid and ethylene, even developmental processes were significantly enriched in GO enrichment analysis of cold and heat stress in biological process term. Several transcription factors (TFs), including MYB66, CBF2, bHLH96and bZIP7 take a pivotal role in response to temperature stresses. Furthermore, heat shock proteins were identified under cold and heat stress, respectively, suggesting these genes participate in reducing cold and heat stress detrimental effect by interacting with TFs or other genes related to abiotic stresses, chlorophyll and photosynthesis, osmoprotectants, and phytohormone as well. Conclusion: This study not only underlying B. platyphylla’s molecular mechanism in response to temperature stresses but also provides candidate genes involved in response to temperature stresses.

RESUMEN Las estructuras de deformación de sedimentos blandos de la Formación Yanchang del Triásico Superior están extendidas lateralmente en la cuenca Ordos. En el área Huachi-Qingyang de esta cuenca, el miembro de petróleo Chang 6 está entre los estratos petrolíferos más significativos del Mesozoico. Este se caracteriza por el desarrollo de cuerpos de arena en el yacimiento. Durante la evolución deposicional del miembro Chang 6, las estructuras de deformación de sedimentos blandos se desarrollaron en el área Huachi-Qingyang, en el medio de la cuenca, inducidas por eventos paleosísmicos. Las estructuras de deformación de sedimentos blandos que se desarrollaron en los cuerpos de arenas del miembro de petróleo Chang 6 son principalmente estructuras esféricas y acojinadas, estructuras plegadas, canales de arena, estratificaciones irregulares enrolladas y fallas sinsedimentarias. La arquitectura de los cuerpos arenosos son el resultado de eventos paleosísmicos y la caída por gravedad; se reconocen principalmente dos tipos de estructuras: 1. Estructuras de deformación de sedimentos blandos por eventos paleosísmicos con cuerpos arenosos del frente del delta. 2. Estructuras de deformación de sedimentos blandos traídas por eventos paleosísmicos con turbiditas (formadas por la caída del frente del delta y redistribuidas debido a la acción sísmica). Como consecuencia, se establecieron los modelos genéticos de los cuerpos arenosos formados por diferentes procesos de sedimentación.

ABSTRACT Soft-sediment deformation (SSD) structures of the Upper Triassic Yanchang Formation are laterally widespread in the Ordos Basin. In the Huachi-Qingyang (H-Q) area of the Ordos Basin, the Chang 6 oil member of the Upper Triassic Yanchang Formation is among the most significant Mesozoic oil-bearing strata. It is characterized by the development of reservoir sand bodies. During the depositional evolution of the Chang 6 oil member, SSD structures induced by paleo-seismic events developed in the H-Q area in the middle of the basin. The SSD structures developed in the sand bodies of the Chang 6 oil member are mainly ball-and-pillow structures, fold structures, sand dikes, irregular convolute stratifications, and synsedimentary faults. The architecture of the sand bodies resulted from paleo-seismic events and gravity slumping and mainly include two types of structures: i) SSD structures driven by paleo-seismic events with delta front sand body (SD-SSD) and ii) SSD structures driven by paleo-seismic events with turbidites (formed by delta-front slumping and re-distribution due to seismic action) (ST-SSD). As a consequence, genetic models of the sand bodies formed by different sedimentation processes are established.

An efficient, convenient and inexpensive CuI-catalyzed method for the synthesis of 1,2-dichlorovinylphosphine oxides from substituted phosphites, diphenylphosphine oxide and 1,2-dichloroethyne has been developed in moderate-good yields, and the reactions provided mainly regioselective anti addition products.

Neste trabalho descreve-se a preparação de seis corantes cianina dimetina trinucleares com núcleos de piridina, obtidos a partir da condensação de iodeto de trimetilpiridínio com aldeídos aromáticos heterocíclicos. As propriedades de absorção e fluorescência dos corantes foram estudadas em solventes com polaridades diferentes. O deslocamento hipsocrômico dos máximos de absorção dos corantes foi observado com o aumento da polaridade do solvente. Foram estudadas as propriedades fluorescentes dos corantes em solução e na presença de DNA. Foi observado um aumento significativo do rendimento quântico de fluorescência na presença de DNA para quatro corantes.. Em particular, um dos corantes emite fluorescência fraca no tampão Tris-HCl, apresentando contudo fluorescência intensa na presença de DNA.

The preparation of six trinucleus dimethine cyanine dyes with pyridine nucleus obtained by the condensation of trimethylpyridinium iodides with heterocyclic aromatic aldehyde was described. The absorption and fluorescence properties of the dyes were studied in different polarity solvents. Blue shift of the maxima absorption of the dyes was observed with the increase of solvents polarity. The fluorescence properties of the dyes in solution and in presence of DNA were studied. Significant enhancement of the fluorescent quantum yield was observed in four dyes in the presence of DNA. Specially, one of six dyes emitted weak fluorescence in Tris-HCl buffer, but displayed bright fluorescence in the presence of DNA.

Anhydrous p-aminobenzene sulphonic acid mediated solvent-free protocol is described for the synthesis of dihydropyrimidi-nones by the cyclocondensation of aldehydes, β-ketoesters and urea/thiourea. Yields obtained are significantly higher than those obtained utilizing classical Biginelli reaction conditions.